Exploring the diversity of promoter and 5’UTR sequences in ancestral, historic and modern wheat

A dataset of promoter and 5’UTR sequences of homoeo-alleles of 495 wheat genes that contribute to agriculturally important traits in 95 ancestral and commercial wheat cultivars is presented here. The high stringency myBaits technology used made individual capture of homoeo-allele promoters possible, which is reported here for the first time. Promoters of most genes are remarkably conserved across the 82 hexaploid cultivars used with <7 haplotypes per promoter and 21% being identical to the reference Chinese Spring. InDels and many high-confidence SNPs are located within predicted plant transcription factor binding sites, potentially changing gene expression. Most haplotypes found in the Watkins landraces and a few haplotypes found in T. monococcum, germplasms hitherto not thought to have been used in modern wheat breeding, are already found in many commercial hexaploid wheats. The full dataset which is useful for genomic and gene function studies and wheat breeding is available at https://rrescloud.rothamsted.ac.uk/index.php/s/3vc9QopcqYEbIUs/authenticate

The H.E.S.S. View of the Central 200 Parsecs

The inner few hundred parsecs of our galaxy provide a laboratory for the study of the production and propagation of energetic particles. Very-high-energy gamma-rays provide an effective probe of these processes and, especially when combined with data from other wave-bands, gamma-rays observations are a powerful diagnostic tool. Within this central region, data from the H.E.S.S. instrument have revealed three discrete sources of very-high-energy gamma-rays and diffuse emission correlated with the distribution of molecular material. Here I provide an overview of these recent results from H.E.S.S.Comment: Proceedings of the Galactic Centre Workshop 200

Proteinaceous effector discovery and characterisation in filamentous plant pathogens

The complicated interplay of plant-pathogen interactions occurs on multiple levels as pathogens evolve to constantly evade the immune responses of their hosts. Many economically important crops fall victim to filamentous pathogens which produce small proteins called effectors to manipulate the host and aid infection/colonisation. Understanding the effector repertoires of pathogens is facilitating an increased understanding of the molecular mechanisms underlying virulence as well as guiding the development of disease control strategies. The purpose of this review is to give a chronological perspective on the evolution of the methodologies used in effector discovery from physical isolation and in silico predictions, to functional characterisation of the effectors of filamentous plant pathogens and identification of their host targets

HESS J1507-622: an unique unidentified source off the Galactic Plane

Galactic very high energy (VHE, > 100 GeV) gamma ray sources in the inner Galaxy H.E.S.S. survey tend to cluster within 1 degree in latitude around the Galactic plane. HESS J1507-622 instead is unique, since it is located at latitude of ~3.5 degrees. HESS J1507-622 is slightly extended over the PSF of the instrument and hence its Galactic origin is clear. The search for counterparts in other wavelength regimes (radio, infrared and X-rays) failed to show any plausible counterparts; and given its position off the Galactic plane and hence the absorption almost one order of magnitude lower, it is very surprising to not see any counterparts especially at X-rays wavelengths (by ROSAT, XMM Newton and Chandra). Its latitude implies that it is either rather close, within about 1 kpc, or is located well off the Galactic plane. And also the models reflect the uniqueness of this object: a leptonic PWN scenario would place this source due to its quite small extension to multi-kpc distance whereas a hadronic scenario would preferentially locate this object at distances of < 1 kpc where the density of target material is higher

Apoplastic and vascular defences

The apoplast comprises the intercellular space between cell membranes, includes the xylem, and extends to the rhizoplane and the outer surfaces of the plant. The apoplast plays roles in different biological processes including plant immunity. This highly specialised space is often the first place where pathogen recognition occurs, and this then triggers the immune response. The immune response in the apoplast involves different mechanisms that restrict pathogen infection. Among these responses, secretion of different molecules like proteases, proteins related to immunity, small RNAs and secondary metabolites play important and often additive or synergistic roles. In addition, production of reactive oxygen species occurs to cause direct deleterious effects on the pathogen as well as reinforce the plant’s immune response by triggering modifications to cell wall composition and providing additional defence signalling capabilities. The pool of available sugar in the apoplast also plays a role in immunity. These sugars can be manipulated by both interactors, pathogens gaining access to nutrients whilst the plant’s responses restrict the pathogen’s access to nutrients. In this review, we describe the latest findings in the field to highlight the importance of the apoplast in plant – pathogen interactions and plant immunity. We also indicate where new discoveries are needed